Lihlahisoa
Eggs Drop Syndrome1976 kokoana-hloko Antibody ELISA Ki
Eggs Drop Syndrome1976 kokoana-hloko Antibody ELISA Ki
| Kakaretso | Use ho lemoha antibody e khethehileng khahlano le EDS76 ka serum ka boleng. |
| Molao-motheo | The Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit e sebelisoa ho lemoha antibody e itseng khahlano le EDS76 ka serum ka boleng.Bakeng sa ho shebella li-antibody ka mor'a tlhahlobo ea EDS76 ea ho itšireletsa mafung le ea serological ea tšoaetso ho Avian. |
| Lipheo tsa ho lemoha | Aantibody khahlano le EDS76 ka serum |
| Mohlala | Serum
|
| Bongata | 1 kit = 192 Teko |
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Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Boitsebiso
Egg Drop Syndrome (EDS-76) ke lefu le tšoaetsanoang le matla le bakoang ke adenovirus sehlopha sa III sa Adenoviridae Avian Virus genus le hemagglutination.Libakeng tse ling tsa lihoai tsa likhoho, tlhahiso ea mahe a mangata a likhoho e ile ea fokotseha ka tšohanyetso, ’me mahe a holofetseng a kang a khetla e bonolo, mahe a se nang likhetla le mahe a likhetla tse tšesaane a ile a hlahisoa ka nako e le ’ngoe.Nako eohle ea lefu lena e nka libeke tse 5-6, ka mor'a moo tekanyo ea tlhahiso ea lehe e nyoloha butle-butle, empa ho thata ho fihla boemong pele ho fokotseha.
Molao-motheo oa Teko
Letlapa lena le sebelisa mokhoa o sa tobang oa ELISA, antigen ea EDS76 e hloekisitsoeng e koahetsoe pele ho likotopo tsa seliba sa enzyme.,haeba ho na le EDS76 e khethehileng ea kokoana-hloko, e tla kopana le antigen e kentsoeng pele, e lahle anti-antibody e sa kopaneng le likarolo tse ling ka ho hlatsoa;ebe o eketsa enzyme e labled anti-EDS76 virus monoclonal antibody, ebe motsoako oa monoclonal antibody le antigen e koahetsoeng pele;lahla conjugate ea enzyme e sa kopanngoeng ka ho hlatsoa;Eketsa substrate ea TMB lilibeng tse nyane, lets'oao le leputsoa ka Enzyme catalysis le ka har'a karolo e fapaneng ea li-antibody ka sampole, sebelisa sebali sa ELISA ho 450nm wavelenth ho lekanya monko oa boleng ba liliba tsa karabelo kamora ho eketsa tharollo ea ho emisa.
ho emisa karabelo.
Litaba
| Reagent | Bolumo 96 Liteko/192Liteko | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Taelo | 1 likhomphutha |
