Kakaretso | Ho sibolloa ha sesole sa 'mele se khethehileng sa Bovine Tuberculosis(BTB). |
Molao-motheo | Setsi sa ho hlahloba lefuba la Bovine Tuberculosis(BTB) Elisa se ka sebelisoa ho bona sethibela-mafu sa Bovine Tuberculosis serum kapa plasma ea Bovine. |
Lipheo tsa ho lemoha | Sethibela-mafu sa Bovine Tuberculosis(BTB). |
Mohlala | Serum
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Bongata | 1 kit = 192 Teko |
Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Mycobacterium bovis e hola butle (nako ea moloko oa lihora tse 16 ho isa ho tse 20) bacterium ea aerobic le sesosa se bakang lefuba likhomong (tse tsejoang e le bovine TB).E amana le Mycobacterium tuberculosis, baktheria e bakang lefuba bathong.M. bovis e ka tlola mokoallo oa mefuta-futa 'me ea baka tšoaetso e kang lefuba ho batho le liphoofolong tse ling tse anyesang.
Sena kit tshebediso e sa tobang ELISA mokhoa, hloekisitsoe BTB antigen is e koahetseng pele on enzyme hantle - micro maqephe. Ha u etsa liteko, eketsa hlapollotsoe serum mohlala, kamora ho qhoqhoa, if Mono is BTB itseng antibody, it thato kopanya ka the e koahetseng pele antigen, lahla the e sa kopanngoeng antibody le tse ding dikarolo ka ho hlatsoa; ebe eketsa enzyme conjugate, lahla the e sa kopanngoeng enzyme conjugate ka
ho hlatsoa. Kenya substrate ea TMB lilibeng tse nyane, lets'oao le leputsoa ka Enzyme catalysis ke ka kotloloho karolo ea li-antibody ka sampuling.
Reagent | Bolumo 96 Liteko/192Liteko | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Taelo | 1 likhomphutha |