Kakaretso | Ho fumanoa ha Li-Antibody tse khethehileng tsa mafu a Newcastle |
Molao-motheo | Sethibela-mafu sa lefu la Newcastle Elisa kit e sebelisoa ho bona sethibela-mafu se ikhethileng khahlanong le lefu la Newcastle Kokoana-hloko (NDV) ka serum, bakeng sa ho beha leihlo antibody ka mor'a NDV immune immunele tlhahlobo ea serological ea tšoaetso ho Avian.
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Lipheo tsa ho lemoha | Antibody ea lefu la Newcastle |
Mohlala | Serum
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Bongata | 1 kit = 192 Teko |
Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Lefu la Newcastle ke lefu le tšoaetsanoang la linonyana tsa linonyana le amang mefuta e mengata ea linonyana tsa lapeng le tse hlaha;e fetisetsoa ho batho.Le hoja e ka tšoaetsa batho, maemong a mangata ha e na matšoao;ke ka seoelo e ka bakang feberu e fokolang le matšoao a kang a ntaramane le/kapa conjunctivitis bathong.Liphello tsa eona li bonahala haholo ho likhoho tsa malapeng ka lebaka la ho ba bonolo haholo le monyetla oa ho ba le liphello tse matla tsa epizootic liindasteri tsa likhoho.E atile linaheng tse ngata.Ha ho na phekolo e tsejoang, empa tšebeliso ea liente tsa prophylactic le mehato ea bohloeki e fokotsa menyetla ea ho qhoma.
Sesebelisoa sena se sebelisa thibela mokhoa oa ELISA, antigen ea NDV e koahetsoe pele ho microplate.Ha u etsa liteko, eketsa sampuli ea serum e hlapolotsoeng, ka mor'a ho kenngoa, haeba ho na le NDV anti-antigen e khethehileng, e tla kopana le antigen e koahetsoeng pele, e lahle anti-antibody e sa kopantsoeng le likarolo tse ling ka ho hlatsoa;ebe u eketsa enzyme e ngotsoeng anti-NDV monoclonal antibody, antibody ka har'a sampuli block motsoako oa monoclonal antibody le antigen e koahetsoeng pele;lahla enzyme e sa kopanngoeng e kopanang le ho hlatsoa. Eketsa karoloana ea TMB lilibeng tse nyane, lets'oao le leputsoa ka Enzyme catalysis le ka har'a karolo e fapaneng ea li-antibody tsa sampole.
Reagent | Bolumo 96 Liteko/192Liteko | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Taelo | 1 likhomphutha |