Nomoro ea lethathamo | RC-CF31 |
Kakaretso | Ho fumanoa ha Canine Dirofilaria immitis antigens, Anaplasma antibodies, E. canis antibodies le LSH antibodies nakong ea metsotso e 10 |
Molao-motheo | Teko ea immunochromatographic ea mohato o le mong |
Lipheo tsa ho lemoha | CHW Ag : Dirofilaria immitis antigens Anapalsma Ab : Anaplasma antibodiesE. canis Ab : Li-antibodies tsa E. canis LSH Ab : L. chagasi, L. infantum, le L. donovani likokoana-hloko |
Mohlala | Mali a Canine a Felletseng, Plasma kapa Serum |
Nako ea ho bala | Metsotso e 10 |
Bongata | Lebokose le 1 (kit) = lisebelisoa tse 10 (ho paka ka motho ka mong) |
Litaba | Kit ea liteko, botlolo ea Buffer, le dropper e ka lahloang |
Polokelo | Mocheso oa Kamore (ho 2 ~ 30℃) |
Ho felloa ke nako | Likhoeli tse 24 ka mor'a tlhahiso |
Tlhokomeliso | Sebelisa nakong ea metsotso e 10 ka mor'a ho bulaSebelisa sampole e loketseng (0.01 ml ea dropper) Sebelisa kamora metsotso e 15-30 ho RT haeba li bolokiloe tlas'a maemo a batang Nka liphetho tsa liteko e le tse fosahetseng kamora metsotso e 10 |
Li-heartworms tsa batho ba baholo li hōla ka bolelele ba lisenthimithara tse 'maloa 'me li lula methapong ea matšoafo moo li ka fumanang limatlafatsi tse lekaneng.Liboko tsa pelo tse ka har'a methapo li baka ho ruruha le ho etsa hematoma.Ka hona, pelo e lokela ho pompa hangata ho feta pele ha liboko li ntse li eketseha ka palo, li thibela methapo ea mali.
Ha tšoaetso e mpefala (ho na le li-heartworms tse fetang 25 ho ntja ea lik'hilograma tse 18), liboko li kena ka atrium e nepahetseng, li thibela ho phalla ha mali.
Ha palo ea li-heartworms e fihla ho feta 50, li ka lula
li-atrium le li-ventricle.
Ha ntja e tšoaelitsoe ke li- heartworm tse fetang 100 karolong e nepahetseng ea pelo, e lahleheloa ke mosebetsi oa pelo ’me qetellong ea shoa.Sena se bolaeang
Phenomenon e bitsoa "Caval Syndrom".
Ho fapana le likokoana-hloko tse ling, li-heartworms li beha likokoanyana tse nyenyane tse bitsoang microfilaria.Microfilaria e ka har'a monoang e kena ka har'a ntja ha monoang o monya mali ho ntja.Li-heartworms tse khonang ho phela ho moamoheli ka lilemo tse 2 lia shoa haeba li sa fallele ho motho e mong nakong eo.Likokoana-hloko tse lulang ntjang e moimana li ka tšoaetsa lesea le sa tsoa emoloa.
Tlhahlobo ea pele ea li-heartworms e bohlokoa haholo ho li felisa.Li-heartworms li feta mehatong e mengata e kang L1, L2, L3 ho kenyelletsa le sethala sa phetiso ka menoang ho fetoha liboko tsa batho ba baholo.
Microfilaria ka monoang e hola ho ba likokoana-hloko tsa L2 le L3 tse khonang ho tšoaetsa lintja ka mor'a libeke tse 'maloa.Khōlo e itšetlehile ka boemo ba leholimo.Mocheso o motle oa likokoana-hloko o feta 13.9 ℃.
Ha monoang o tšoaelitsoeng o loma ntja, microfilaria ea L3 e phunyeletsa letlalong la eona.Letlalong, microfilaria e hola ho L4 bakeng sa libeke tse 1 ~ 2.Kamora ho lula letlalong ka likhoeli tse 3, L4 e fetoha L5, e kenang maling.
L5 e le mofuta oa pelo ea batho ba baholo e kenang pelong le methapong ea matšoafo moo 5 ~ 7 likhoeli hamorao li-heartworms li beha likokoanyana.
Histori ea lefu lena le lintlha tsa kliniki tsa ntja e kulang, le mekhoa e sa tšoaneng ea ho hlahloba e lokela ho nkoa ha ho hlahlojoa ntja.Ka mohlala, X-ray, ultrasound scan, tlhahlobo ea mali, ho lemoha microfilaria 'me, boemong bo bobe ka ho fetisisa, ho hlokahala hore motho a hlahlojoe.
Tlhahlobo ea serum;
Ho fumanoa ha li-antibodies kapa li-antigen maling
Tlhahlobo ea li-antigen;
Sena se tsepamisitse maikutlo tabeng ea ho lemoha li-antigen tse khethehileng tsa li-heartworms tsa batho ba baholo.Tlhahlobo e etsoa sepetlele 'me katleho ea eona e phahame.Lisebelisoa tsa liteko tse fumanehang 'marakeng li etselitsoe ho lemoha li-heartworms tsa batho ba baholo ba likhoeli tse 7-8 e le hore ho be thata ho lemoha li-heartworms tse ka tlase ho likhoeli tse 5.
Tšoaetso ea heartworms e phekoloa ka katleho maemong a mangata.Ho felisa li-heartworms tsohle, tšebeliso ea meriana ke tsela e molemohali.Ho lemoha kapele ha li-heartworms ho phahamisa sekhahla sa katleho ea phekolo.Leha ho le joalo, qetellong ea ts'oaetso, bothata bo ka hlaha, bo etsa hore phekolo e be thata haholoanyane.
Baktheria Anaplasma phagocytophilum (eo pele e neng e le Ehrilichia phagocytophila) e ka baka tšoaetso mefuteng e mengata ea liphoofolo ho kenyeletsa le batho.Lefu lena la liphoofolo tse rumineng malapeng le boetse le bitsoa tick-borne fever (TBF), 'me le tsebile bonyane lilemo tse 200.Likokoana-hloko tsa lelapa la Anaplasmataceae li na le gram-negative, nonmotile, coccoid ho ea ho li-ellipsoid tse phelang, tse fapaneng ka boholo ho tloha ho 0.2 ho isa ho 2.0um bophara.Ke li-aerobes tse tlamang, ha li na tsela ea glycolytic, 'me kaofela ke likokoana-hloko tse tlamang ka hare ho lisele.Mefuta eohle ea mofuta oa Anaplasma e lula ka har'a li-vacuoles tse nang le membrane ka har'a lisele tse sa holang kapa tse holileng tsa hematopoietic tsa liphoofolo tse anyesang.Phagocytophilum e tšoaetsa li-neutrophils 'me lentsoe granulocytotropic le bolela li-neutrophils tse tšoaelitsoeng.Lintho tse phelang li fumanoa seoelo ho li-eosinophils.
Anaplasma phagocytophilum
Matšoao a tloaelehileng a kliniki acanine anaplasmosis kenyeletsa feberu e phahameng, lethargy, ho tepella maikutlo le polyarthritis.Matšoao a methapo ea kutlo (ataxia, seizures le bohloko ba molala) le ona a ka bonoa.Anaplasma phagocytophilum tšoaetso ha se hangata e bolaeang ntle le haeba e rarahane ke mafu a mang.Litahlehelo tse tobileng, maemo a holofetseng le tahlehelo ea tlhahiso li 'nile tsa bonoa likonyana.Ho ntšoa ha mpa le ho senyeha ha spermatogenesis ho linku le likhomo ho tlalehiloe.Bohloko ba tšoaetso bo susumetsoa ke lintlha tse 'maloa, tse kang mefuta e fapaneng ea Anaplasma phagocytophilum e amehang, likokoana-hloko tse ling, lilemo, boemo ba ho itšireletsa mafung le boemo ba moeti, le lintlha tse kang boemo ba leholimo le tsamaiso.Ho lokela ho boleloa hore liponahatso tsa kliniki ho batho li fapana ho tloha ho bokuli bo bonolo bo ikemetseng bo kang feberu, ho isa ho ts'oaetso e behang bophelo kotsing.Leha ho le joalo, mafu a mangata a batho a ka 'na a fella ka matšoao a fokolang kapa a se nang letho.
Anaplasma phagocytophilum e fetisoa ke liboseleise tsa ixodid.United States, likokoana-hloko tse ka sehloohong ke Ixodes scapularis le Ixodes pacificus, ha Ixode ricinus e fumanoe e le eona ntho e ka sehloohong e ntšang likokoana-hloko Europe.Anaplasma phagocytophilum e fetisoa ka mokhoa oa transstadially ke li-ticks tsena tsa vector, 'me ha ho na bopaki ba phetiso ea transovarial.Liphuputso tse ngata ho fihlela joale tse entseng lipatlisiso ka bohlokoa ba liphoofolo tse anyesang tsa A. phagocytophilum le likokoana-hloko tsa eona tsa tick li tsepamisitse maikutlo ho litoeba empa phoofolo ena e na le mefuta e mengata ea liphoofolo tse anyesang, e tšoaetsang likatse tse ruuoang lapeng, lintja, linku, likhomo le lipere.
Indirect immunofluorescence assay ke teko e ka sehloohong e sebelisetsoang ho bona tšoaetso.Mehlala ea serum ea acute le convalescent phase e ka hlahlojoa ho sheba phetoho e menahaneng hane ea antibody titer ho Anaplasma phagocytophilum.Li-intracellular inclusions (morulea) li bonoa ka li-granulocyte ho Wright kapa Gimsa stained blood smears.Mekhoa ea polymerase chain reaction(PCR) e sebelisoa ho fumana Anaplasma phagocytophilum DNA.
Ha ho na ente e teng ho thibela tšoaetso ea Anaplasma phagocytophilum.Thibelo e itšetlehile ka ho qoba ho pepeseha ho vector ea tick (Ixodes scapularis, Ixodes pacificus, le Ixode ricinus) ho tloha nakong ea selemo ho fihlela ka hoetla, tšebeliso ea prophylatic ea li-anticaricides, le tšebeliso ea prophylactic ea doxycycline kapa tetracycline ha u etela Ixodes scapularis, Ixodes pacide-ricinus le Ixodes pacide-ricinus libaka tse sa feleng.
Ehrlichia canis ke dikokwana-hloko tse nyane tse sebopeho sa molamu tse fetiswang ke letshwao la ntja e sootho, Rhipicephalus sanguineus.E. canis ke sesosa sa classical ehrlichiosis ho lintja.Lintja li ka tšoaetsoa ke Ehrlichia spp tse ngata.empa e tloaelehileng ka ho fetisisa e bakang canine ehrlichiosis ke E. canis.
E. canis e se e tsejoa hore e hasane hohle United States, Europe, Amerika Boroa, Asia le Mediterranean.
Lintja tse tšoaelitsoeng tse sa phekoloeng li ka 'na tsa se ke tsa jara lefu lena ka lilemo tse ngata 'me qetellong tsa shoa ka lebaka la tšollo ea mali e ngata.
Tšoaetso ea Ehrlichia canis ho lintja e arotsoe ka mekhahlelo e 3;
ACUTE PHASE: Ena ka kakaretso ke mohato o bobebe haholo.Ntja e tla be e hloname, e se na lijo, 'me e ka 'na eaba e atolositse lymph nodes.Ho ka 'na ha e-ba le feberu hape empa ke ka seoelo mokhahlelo ona o bolaeang ntja.Ba bangata ba hlakisa 'mele ka bobona empa ba bang ba tla fetela mohatong o latelang.
SUBCLINICAL PHASE: Karolong ena, ntja e bonahala e tloaelehile.Sebōpuoa se itšoere ka har'a spleen 'me ha e le hantle se ipatile ka ntle.
MOHATO OA BOPHELO: Mokhahlelong ona ntja ea boela ea kula.Ho fihla ho 60% ea lintja tse tšoaelitsoeng ke E. canis li tla tsoa mali ka mokhoa o sa tloaelehang ka lebaka la ho fokotseha ha lipalo tsa liplatelete.Ho ruruha ho tebileng mahlong a "uveitis" ho ka hlaha ka lebaka la ts'usumetso ea nako e telele ea 'mele ea ho itšireletsa mafung.Liphello tsa methapo ea kutlo le tsona li ka bonoa.
Tlhahlobo e hlakileng ea Ehrlichia canis e hloka pono ea morula ka har'a monocyte ka cytology, ho fumanoa ha E. canis serum antibodies ka tlhahlobo e sa tobang ea immunofluorescence antibody test (IFA), polymerase chain reaction (PCR) amplification, le/kapa blotting ea gel (Western immunoblotting).
Ntho e ka sehloohong ea thibelo ea canine ehrlichiosis ke ho laola tick.Moriana oa khetho bakeng sa phekolo ea mefuta eohle ea ehrlichiosis ke doxycycline bonyane khoeli e le 'ngoe.Ho lokela ho ba le ntlafatso e kholo ea kliniki nakong ea lihora tse 24-48 kamora ho qala kalafo ho lintja tse nang le lefu le matla kapa le bobebe le sa foleng.Nakong ena, palo ea liplatelete e qala ho eketseha 'me e lokela ho ba e tloaelehileng matsatsing a 14 ka mor'a ho qala kalafo.
Ka mor'a tšoaetso, hoa khoneha ho tšoaetsoa hape;ho itšireletsa mafung ha ho tšoarelle ka mor'a tšoaetso e fetileng.
Thibelo e ntle ka ho fetisisa ea ehrlichiosis ke ho boloka lintja li se na liboseleise.Sena se lokela ho kenyelletsa ho hlahloba letlalo letsatsi le leng le le leng bakeng sa liboseleise le ho phekola lintja ka taolo ea liboseleise.Kaha liboseleise li na le mafu a mang a kotsi, a kang lefu la Lyme, anaplasmosis le Rocky Mountain spotted fever, ke habohlokoa ho boloka lintja li se na liboseleise.
Leishmaniasis ke lefu le leholo le le matla la likokoana-hloko ho batho, li-canine le li-felines.Moemeli oa leishmaniasis ke kokoana-hloko ea protozoa 'me ke ea leishmania donovani complex.Kokoana-hloko ena e ajoa haholo linaheng tse futhumetseng le tsa tropike tsa Europe Boroa, Afrika, Asia, Amerika Boroa le Amerika Bohareng.Leishmania donovani infantum (L. infantum) e ikarabella bakeng sa lefu la feline le canine Europe Boroa, Afrika le Asia.Canine Leishmaniasis ke lefu le matla le tsoelang pele la tsamaiso.Ha se lintja tsohle tse nang le lefu la kliniki ka mor'a ho entoa le likokoana-hloko.Tsoelo-pele ea lefu la kliniki e itšetlehile ka mofuta oa karabelo ea 'mele ea ho itšireletsa mafung eo liphoofolo ka bomong li nang le eona
khahlanong le likokoana-hloko.
Ka Canine
Lipontšo tse peli tsa visceral le cutaneous li ka fumanoa ka nako e le 'ngoe ho lintja;ho fapana le batho, li-syndromes tse arohaneng tsa cutaneous le visceral ha li bonoe.Matšoao a kliniki a fapane 'me a ka etsisa mafu a mang.Tšoaetso ea asymptomatic le eona e ka hlaha.Matšoao a tloaelehileng a visceral a ka kenyelletsa feberu (e ka bang nako le nako), phokolo ea mali, lymphadenopathy, splenomegaly, lethargy, ho fokotseha ha mamello ea boikoetliso, ho theola boima ba 'mele le ho fokotseha ha takatso ea lijo.Matšoao a sa tloaelehang a visceral a kenyelletsa letšollo, ho hlatsa, melena, glomerulonephritis, ho hloleha ha sebete, epistaxis, polyuria-polydipsia, ho thimola, ho holofala (ka lebaka la polyarthritis kapa myositis), ascites, le lefu la ho ruruha le sa foleng.
Ho Feline
Hase hangata likatse li nang le tšoaetso.Likatseng tse ngata tse tšoaelitsoeng, liso li lekanyelitsoe feela ho liso tsa letlalo tse kolobisitsoeng, tseo hangata li fumanoang molomong, nkong, lintšing tsa mahlo, kapa pinnae.Maqeba a Visceral le matšoao ha a fumanehe.
Potoloho ea bophelo e phethoa ka lihlopha tse peli.Sebōpuoa se nang le lesapo la mokokotlo le se se nang lesapo la mokokotlo (ntsintsi ea lehlabathe).Ntsintsi ya lehlabathe e tshehadi e iphepa ka diphoofolo tse nang le lesapo la mokokotlo leho ja amastigotes.Likokoana-hloko tsa promastigote li hlaha ka har'a kokoanyana.Li-promastigote li kenngoa ka har'a phoofolo e nang le lesapo la mokokotlo nakong ea ho fepa santa.Li-promastigotes li fetoha li-amastigotes 'me li ata haholo-holo ho macrophages.Ho ata ka har'a macrophages ea letlalo, mucosa le viscera, ho baka leishmaniasis ea letlalo, lesapo la mokokotlo le visceral leishmaniasis ka ho latellana.
Ho lintja, leishmaniasis e atisa ho fumanoa ka ho shebella likokoana-hloko ka ho toba, ka ho sebelisa Giemsa kapa matheba a potlakileng, ho smears ho tloha lymph node, spleen, kapa masapo a masapo a masapo, li-tissue biopsies, kapa maqeba a letlalo a tsoang liso.Likokoana-hloko li ka boela tsa fumanoa liso tsa mahlo, haholo-holo ka granulomas.Li-amastigote li chitja ho isa ho li-oval parasite, tse nang le khubu e chitja ea basophilic le kinetoplast e nyane e kang molamu.Li fumaneha ka har'a macrophages kapa li lokollotsoe liseleng tse phatlohileng.Mekhoa ea Immunohistochemistry le polymerase chain reaction (PCR) le eona e sebelisoa.
Lithethefatsi tse sebelisoang ka ho fetisisa ke: Meglumine Antimoniate e amanang le Allopurinol, Aminosidine, 'me morao tjena, Amphotericin B. Lithethefatsi tsena kaofela li hloka mekhoa e mengata ea litekanyetso, 'me sena se tla itšetleha ka boemo ba mokuli le tšebelisano ea mong'a eona.Ho khothalletsoa hore phekolo ea tlhokomelo e bolokehe ka allopurinol, hobane ho ke ke ha khoneha ho etsa bonnete ba hore lintja li ke ke tsa khutlela morao haeba phekolo e khaotsa.Tšebeliso ea li-collar tse nang le chefo e bolaeang likokoanyana, li-shampoo kapa li-sprays tse sebetsang hantle ho sireletsa lintja ho longoa ke lehlabathe li tlameha ho tsoela pele ho sebelisoa ho bakuli bohle ba tlas'a kalafo.Taolo ea li-vector ke e 'ngoe ea likarolo tsa bohlokoa tsa taolo ea mafu.
Sandfly e kotsing ea ho hlaseloa ke likokoanyana tse tšoanang le tsa kokoana-hloko ea malaria.