Nomoro ea lethathamo | RC-CF29 |
Kakaretso | Ho fumanoa ha Canine Dirofilaria immitis antigens, Anaplasma antibodies, E. canis antibodies nakong ea metsotso e 10 |
Molao-motheo | Teko ea immunochromatographic ea mohato o le mong |
Lipheo tsa ho lemoha | CHW Ag : Dirofilaria immitis antigens Anapalsma Ab : Anaplasma antibodiesE. canis Ab : Li-antibodies tsa E. canis |
Mohlala | Mali a Canine a Felletseng, Plasma kapa Serum |
Nako ea ho bala | Metsotso e 10 |
Bongata | Lebokose le 1 (kit) = lisebelisoa tse 10 (ho paka ka motho ka mong) |
Litaba | Kit ea liteko, botlolo ea Buffer, le dropper e ka lahloang |
Polokelo | Mocheso oa Kamore (ho 2 ~ 30℃) |
Ho felloa ke nako | Likhoeli tse 24 ka mor'a tlhahiso |
Tlhokomeliso | Sebelisa nakong ea metsotso e 10 ka mor'a ho bulaSebelisa sampole e loketseng (0.01 ml ea dropper) Sebelisa kamora metsotso e 15-30 ho RT haeba li bolokiloe tlas'a maemo a batang Nka liphetho tsa liteko e le tse fosahetseng kamora metsotso e 10 |
Li-heartworms tsa batho ba baholo li hōla ka bolelele ba lisenthimithara tse 'maloa 'me li lula methapong ea matšoafo moo li ka fumanang limatlafatsi tse lekaneng.Liboko tsa pelo tse ka har'a methapo li baka ho ruruha le ho etsa hematoma.Ka hona, pelo e lokela ho pompa hangata ho feta pele ha liboko li ntse li eketseha ka palo, li thibela methapo ea mali.
Ha tšoaetso e mpefala (ho na le li-heartworms tse fetang 25 ho ntja ea lik'hilograma tse 18), liboko li kena ka atrium e nepahetseng, li thibela ho phalla ha mali.
Ha palo ea li-heartworms e fihla ho feta 50, li ka lula
li-atrium le li-ventricle.
Ha ntja e tšoaelitsoe ke li- heartworm tse fetang 100 karolong e nepahetseng ea pelo, e lahleheloa ke mosebetsi oa pelo ’me qetellong ea shoa.Sena se bolaeang
Phenomenon e bitsoa "Caval Syndrom".
Ho fapana le likokoana-hloko tse ling, li-heartworms li beha likokoanyana tse nyenyane tse bitsoang microfilaria.Microfilaria e ka har'a monoang e kena ka har'a ntja ha monoang o monya mali ho ntja.Li-heartworms tse khonang ho phela ho moamoheli ka lilemo tse 2 lia shoa haeba li sa fallele ho motho e mong nakong eo.Likokoana-hloko tse lulang ntjang e moimana li ka tšoaetsa lesea le sa tsoa emoloa.
Tlhahlobo ea pele ea li-heartworms e bohlokoa haholo ho li felisa.Li-heartworms li feta mehatong e mengata e kang L1, L2, L3 ho kenyelletsa le sethala sa phetiso ka menoang ho fetoha liboko tsa batho ba baholo.
Microfilaria ka monoang e hola ho ba likokoana-hloko tsa L2 le L3 tse khonang ho tšoaetsa lintja ka mor'a libeke tse 'maloa.Khōlo e itšetlehile ka boemo ba leholimo.Mocheso o motle oa likokoana-hloko o feta 13.9 ℃.
Ha monoang o tšoaelitsoeng o loma ntja, microfilaria ea L3 e phunyeletsa letlalong la eona.Letlalong, microfilaria e hola ho L4 bakeng sa libeke tse 1 ~ 2.Kamora ho lula letlalong ka likhoeli tse 3, L4 e fetoha L5, e kenang maling.
L5 e le mofuta oa pelo ea batho ba baholo e kenang pelong le methapong ea matšoafo moo 5 ~ 7 likhoeli hamorao li-heartworms li beha likokoanyana.
Tšoaetso ea heartworms e phekoloa ka katleho maemong a mangata.Ho felisa li-heartworms tsohle, tšebeliso ea meriana ke tsela e molemohali.Ho lemoha kapele ha li-heartworms ho phahamisa sekhahla sa katleho ea phekolo.Leha ho le joalo, qetellong ea ts'oaetso, bothata bo ka hlaha, bo etsa hore phekolo e be thata haholoanyane.
Baktheria Anaplasma phagocytophilum (eo pele e neng e le Ehrilichia phagocytophila) e ka baka tšoaetso mefuteng e mengata ea liphoofolo ho kenyeletsa le batho.Lefu lena la liphoofolo tse rumineng malapeng le boetse le bitsoa tick-borne fever (TBF), 'me le tsebile bonyane lilemo tse 200.Likokoana-hloko tsa lelapa la Anaplasmataceae li na le gram-negative, nonmotile, coccoid ho ea ho li-ellipsoid tse phelang, tse fapaneng ka boholo ho tloha ho 0.2 ho isa ho 2.0um bophara.Ke li-aerobes tse tlamang, ha li na tsela ea glycolytic, 'me kaofela ke likokoana-hloko tse tlamang ka hare ho lisele.Mefuta eohle ea mofuta oa Anaplasma e lula ka har'a li-vacuoles tse nang le membrane ka har'a lisele tse sa holang kapa tse holileng tsa hematopoietic tsa liphoofolo tse anyesang.Phagocytophilum e tšoaetsa li-neutrophils 'me lentsoe granulocytotropic le bolela li-neutrophils tse tšoaelitsoeng.Lintho tse phelang li fumanoa seoelo ho li-eosinophils.
Anaplasma phagocytophilum
Matšoao a tloaelehileng a kliniki a canine anaplasmosis a kenyelletsa feberu e phahameng, ho khathala, ho tepella maikutlong le polyarthritis.Matšoao a methapo ea kutlo (ataxia, seizures le bohloko ba molala) le ona a ka bonoa.Anaplasma phagocytophilum tšoaetso ha se hangata e bolaeang ntle le haeba e rarahane ke mafu a mang.Litahlehelo tse tobileng, maemo a holofetseng le tahlehelo ea tlhahiso li 'nile tsa bonoa likonyana.Ho ntšoa ha mpa le ho senyeha ha spermatogenesis ho linku le likhomo ho tlalehiloe.Bohloko ba tšoaetso bo susumetsoa ke lintlha tse 'maloa, tse kang mefuta e fapaneng ea Anaplasma phagocytophilum e amehang, likokoana-hloko tse ling, lilemo, boemo ba ho itšireletsa mafung le boemo ba moeti, le lintlha tse kang boemo ba leholimo le tsamaiso.Ho lokela ho boleloa hore liponahatso tsa kliniki ho batho li fapana ho tloha ho bokuli bo bonolo bo ikemetseng bo kang feberu, ho isa ho ts'oaetso e behang bophelo kotsing.Leha ho le joalo, mafu a mangata a batho a ka 'na a fella ka matšoao a fokolang kapa a se nang letho.
Anaplasma phagocytophilum e fetisoa ke liboseleise tsa ixodid.United States, likokoana-hloko tse ka sehloohong ke Ixodes scapularis le Ixodes pacificus, ha Ixode ricinus e fumanoe e le eona ntho e ka sehloohong e ntšang likokoana-hloko Europe.Anaplasma phagocytophilum e fetisoa ka mokhoa oa transstadially ke li-ticks tsena tsa vector, 'me ha ho na bopaki ba phetiso ea transovarial.Liphuputso tse ngata ho fihlela joale tse entseng lipatlisiso ka bohlokoa ba liphoofolo tse anyesang tsa A. phagocytophilum le likokoana-hloko tsa eona tsa tick li tsepamisitse maikutlo ho litoeba empa phoofolo ena e na le mefuta e mengata ea liphoofolo tse anyesang, e tšoaetsang likatse tse ruuoang lapeng, lintja, linku, likhomo le lipere.
Indirect immunofluorescence assay ke teko e ka sehloohong e sebelisetsoang ho bona tšoaetso.Mehlala ea serum ea acute le convalescent phase e ka hlahlojoa ho sheba phetoho e menahaneng hane ea antibody titer ho Anaplasma phagocytophilum.Li-intracellular inclusions (morulea) li bonoa ka li-granulocyte ho Wright kapa Gimsa stained blood smears.Mekhoa ea polymerase chain reaction(PCR) e sebelisoa ho fumana Anaplasma phagocytophilum DNA.
Ha ho na ente e teng ho thibela tšoaetso ea Anaplasma phagocytophilum.Thibelo e itšetlehile ka ho qoba ho pepeseha ho vector ea tick (Ixodes scapularis, Ixodes pacificus, le Ixode ricinus) ho tloha nakong ea selemo ho fihlela ka hoetla, tšebeliso ea prophylatic ea li-anticaricides, le tšebeliso ea prophylactic ea doxycycline kapa tetracycline ha u etela Ixodes scapularis, Ixodes pacide-ricinus le Ixodes pacide-ricinus libaka tse sa feleng.
Ehrlichia canis ke dikokwana-hloko tse nyane tse sebopeho sa molamu tse fetiswang ke letshwao la ntja e sootho, Rhipicephalus sanguineus.E. canis ke sesosa sa classical ehrlichiosis ho lintja.Lintja li ka tšoaetsoa ke Ehrlichia spp tse ngata.empa e tloaelehileng ka ho fetisisa e bakang canine ehrlichiosis ke E. canis.
E. canis e se e tsejoa hore e hasane hohle United States, Europe, Amerika Boroa, Asia le Mediterranean.
Lintja tse tšoaelitsoeng tse sa phekoloeng li ka 'na tsa se ke tsa jara lefu lena ka lilemo tse ngata 'me qetellong tsa shoa ka lebaka la tšollo ea mali e ngata.
Tšoaetso ea Ehrlichia canis ho lintja e arotsoe ka mekhahlelo e 3;
ACUTE PHASE: Ena ka kakaretso ke mohato o bobebe haholo.Ntja e tla be e hloname, e se na lijo, 'me e ka 'na eaba e atolositse lymph nodes.Ho ka 'na ha e-ba le feberu hape empa ke ka seoelo mokhahlelo ona o bolaeang ntja.Ba bangata ba hlakisa 'mele ka bobona empa ba bang ba tla fetela mohatong o latelang.
SUBCLINICAL PHASE: Karolong ena, ntja e bonahala e tloaelehile.Sebōpuoa se itšoere ka har'a spleen 'me ha e le hantle se ipatile ka ntle.
MOHATO OA BOPHELO: Mokhahlelong ona ntja ea boela ea kula.Ho fihla ho 60% ea lintja tse tšoaelitsoeng ke E. canis li tla tsoa mali ka mokhoa o sa tloaelehang ka lebaka la ho fokotseha ha lipalo tsa liplatelete.Ho ruruha ho tebileng mahlong a "uveitis" ho ka hlaha ka lebaka la ts'usumetso ea nako e telele ea 'mele ea ho itšireletsa mafung.Liphello tsa methapo ea kutlo le tsona li ka bonoa.
Tlhahlobo e hlakileng ea Ehrlichia canis e hloka pono ea morula ka har'a monocyte ka cytology, ho fumanoa ha E. canis serum antibodies ka tlhahlobo e sa tobang ea immunofluorescence antibody test (IFA), polymerase chain reaction (PCR) amplification, le/kapa blotting ea gel (Western immunoblotting).
Ntho e ka sehloohong ea thibelo ea canine ehrlichiosis ke ho laola tick.Moriana oa khetho bakeng sa phekolo ea mefuta eohle ea ehrlichiosis ke doxycycline bonyane khoeli e le 'ngoe.Ho lokela ho ba le ntlafatso e kholo ea kliniki nakong ea lihora tse 24-48 kamora ho qala kalafo ho lintja tse nang le lefu le matla kapa le bobebe le sa foleng.Nakong ena, palo ea liplatelete e qala ho eketseha 'me e lokela ho ba e tloaelehileng matsatsing a 14 ka mor'a ho qala kalafo.
Ka mor'a tšoaetso, hoa khoneha ho tšoaetsoa hape;ho itšireletsa mafung ha ho tšoarelle ka mor'a tšoaetso e fetileng.
Thibelo e ntle ka ho fetisisa ea ehrlichiosis ke ho boloka lintja li se na liboseleise.Sena se lokela ho kenyelletsa ho hlahloba letlalo letsatsi le leng le le leng bakeng sa liboseleise le ho phekola lintja ka taolo ea liboseleise.Kaha liboseleise li na le mafu a mang a kotsi, a kang lefu la Lyme, anaplasmosis le Rocky Mountain spotted fever, ke habohlokoa ho boloka lintja li se na liboseleise.