Kakaretso | Ho tsebahatsa e khethehilengMofuta oa A oa Maoto le Molomo antibody |
Molao-motheo | Selwantsha-mogare sa Mofuta wa A sa ELISA se sebediswang ho lemoha dithibela-mafu tsa kokoana-hloko ya lefu la tlhako le molomo ka hara serum ya dikolobe, dikgomo, dinku le dipodi bakeng sa tekolo ya boitshireletso ba moento wa FMD. |
Lipheo tsa ho lemoha | Mofuta oa A Sethibela-mafu sa Maoto le Molomo |
Mohlala | Serum
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Bongata | 1 kit = 192 Teko |
Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Kokoana-hloko ea lefu la maoto le molomo(FMDV) ke eonapathogense bakanglefu la maoto le molomo. Ke apicornavirus, setho sa prototypical sa lelokoAphthovirus.Lefu lena, le bakang li-vesicles (mahlaseli) molomong le maotong alikhomo, likolobe, linku, lipōli, le tse linge nang le tlhako e arohanengliphoofolo li tšoaetsanoa haholo le seoa se seholo satemo ea liphoofolo.
Sethala sena se sebelisa mokhoa oa tlholisano oa ELISA ho li-antigen tsa kokoana-hloko ea lefu la leoto le molomo lilibeng tsa microplate.Ha u etsa liteko, eketsa sampole ea serum e hlapolotsoeng le anti-FMD Ab, ka mor'a ho kenngoa, haeba ho na le anti-antigen ea FMD, e tla kopana le antigen e koahetsoeng pele, antibody e ka har'a sampole thibela motsoako oa anti-FMD anti-antigen le antigen e koahetsoeng pele;lahla conjugate ea enzyme e sa kopanngoeng ka ho hlatsoa;Eketsa karoloana ea TMB lilibeng tse nyane, lets'oao le leputsoa ka Enzyme catalysis le ka har'a karolo e fapaneng ea li-antibody tsa sampole.
Kokoana-hloko ea lefu la maoto le molomoe etsahala ho tse supileng tse kholoserotypes: O, A, C, SAT-1, SAT-2, SAT-3, le Asia-1.Li-serotypes tsena li bonts'a sebaka se itseng, 'me serotype ea O e atile haholo.
Reagent | Bolumo 96 Liteko/192Liteko | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Taelo | 1 likhomphutha |