Lihlahisoa
Chicken Infectious Bursal Virus Ab Elisa kit
Chicken Infectious Bursal Virus Ab Elisa kit
| Kakaretso | Ho sibolloa ha antibody e fokolang khahlanong le bursa e tšoaetsanoang ea Fabricius virus ho serum ea likhoho |
| Lipheo tsa ho lemoha | Antibody ea kokoana-hloko ea lefu la bursal |
| Mohlala | Serum
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| Bongata | 1 kit = 192 Teko |
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Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Boitsebiso
Lefu le tšoaetsanoang la bursal(IBD), e tsejoang hape e le lefu la Gumboro, infectious bursitis le infectious avian nephrosis, ke lefu le tšoaetsanoang haholo ho bacha.likhohole likuku tse bakoang ke infectious bursal disease virus (IBDV), e khetholloang keimmunosuppressionle lefu ka kakaretso nakong ea libeke tse 3 ho isa ho tse 6.Lefu lena le ile la fumanoa ka lekhetlo la pele kaGumboro, Delawareka 1962. Ho bohlokoa moruong indastering ea likhoho lefats'eng ka bophara ka lebaka la ho ba le ts'oaetso ea mafu a mang le tšitiso e mpe ea katleho.ente.Lilemong tsa morao tjena, mefuta e kotsi haholo ea IBDV (vvIBDV), e bakang lefu le matla la likhoho, e hlahile Europe,amerika boroa,Asia Boroa-bochabela, Afrika leBochabela bohareng.Tšoaetso e etsoa ka tsela ea oro-fecal, 'me nonyana e anngoeng ke eona e ntša kokoana-hloko ka nako e ka etsang libeke tse 2 ka mor'a tšoaetso.Lefu lena le fetisoa habonolo ho tloha ho likhoho tse tšoaelitsoeng ho ea ho likhoho tse phetseng hantle ka lijo, metsi, le ho ama 'mele.
Molao-motheo oa Teko
Thepa e sebelisa mokhoa oa tlholisano oa ELISA, kokoana-hloko e tšoaetsanoang pele ho bursal virus VP2 protheine ho microplate, le ho qothisana lehlokoa le anti-VP2 protheine antibody ka serum bakeng sa vector ea mohato o tiileng o sebelisa anti-VP2 protheine monoclonal antibody.Tekong, ho tla etsoa tlhahlobo ea anti-VP2 protheine, 'me ka mor'a ho qhoqhoa, haeba sampole e na le kokoana-hloko e tšoaetsanoang ea bursal virus VP2 protheine-specific antibody, e tlama ho antigen holim'a poleiti e koahetsoeng.Ka tsela eo ho thibela ho tlama ha anti-VP2 protheine monoclonal antibody ho antigen, ka mor'a ho hlatsoa ho tlosa antibody e sa tlangoa le likarolo tse ling;ebe o kenya anti-toeba-anti-mouse-lebeled secondary antibody ho tlama ka ho khetheha ho antigen-anti-antibody complex holim'a poleiti ea ho lemoha;Conjugate ea enzyme e sa tlamisoang e tlosoa ka ho hlatsoa;substrate ea TMB e kenyelelitsoe ho microwell ho hlahisa mmala, 'me boleng ba ho monya ba sampole bo amana hampe le litaba tsa anti-VP2 protheine antibody e ka har'a eona, ka hona ho fihlela sepheo sa ho lemoha anti-VP2 protheine antibody sampoleng.
Litaba
| Reagent | Bolumo 96 Liteko/192Liteko | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Taelo | 1 likhomphutha |
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