Kakaretso | Ho fumanwa ha Selwantsha-mogare sa Maoto le Bolwetsi bja Molomo The BRU |
Molao-motheo | Setsi sa tlhahlobo sa BRU antibody ELISA se sebedisetswa ho lemoha dithibela-mafu tsa Brucellosis serum ya dikolobe, dikgomo, dinku le dipodi. |
Lipheo tsa ho lemoha | Sesole sa 'mele sa BRU |
Mohlala | Serum
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Bongata | 1 kit = 192 Teko |
Botsitso le Polokelo | 1) Li-reagents tsohle li lokela ho bolokoa ho 2 ~ 8 ℃.Se hatsele. 2) Shelf bophelo ke likhoeli tse 12.Sebelisa li-reagents tsohle pele letsatsi la ho felloa ke nako ho kit.
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Sehlopha sa BRU antibodyELISAlisebelisoa tsa tekosebelisa ho ho lemoha li-antibodies tsa Brucellosis ka serum ea likolobe, likhomo, sheep le pōli .
Tšebeliso ea sehlahisoa sena mokhoa oa tlholisano oa ELISA oa ho roala peleed BRU li-antigen tse lilibeng tsa microplate.Ha u etsa liteko, eketsa sampole ea serum e hlapolotsoeng leenzyme e bitsoang anti-BRU monoclonal antibody, ka mor'a ho kenngoa, haeba ho le teng na le BRU antibody, e tla kopana le antigen e koahetsoeng pele, antibody e ka har'a sampole block motsoako oa monoclonal antibody le antigen e koahetsoeng pele;lahla conjugate ea enzyme e sa kopanngoeng ka ho hlatsoa;Kenya karoloana ea TMB lilibeng tse nyane, lets'oao le leputsoa ka Enzyme catalysis le ka har'a karolo e fapaneng ea li-antibody ka har'a sampole..
Reagent | Bolumo 96 Liteko/192Liteko | ||
1 |
| 1ea/2ea | |
2 |
| 2 ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Taelo | 1 likhomphutha |