1.Eketsa reagent ho sampole ea metsi ea 100ml, ka mor'a ho qhibiliha, e kene ka 36 ° C bakeng sa 24h.
2. Tlhaloso ea liphetho:
colorless = mpe
Yellow = e ntle bakeng sa kakaretso ea coliforms
Yellow + fluorescence = Escherichia coli positive.
QUANTlTATlVE DETECTlON
1. Kenya li-reagents ho sampuli ea metsi 'me u kopanye hantle
2. Tšela ka har'a poleiti ea ho lemoha boleng ba seliba se 51 (quantitative well plate) kapa poleiti ea 97-well quantitative discovery plate (quantitative well plate)
3. Sebelisa mochine oa ho tiisa palo o laoloang ke lenaneo
ho tiisa disc ea quantitative discovery (quantitative well plate) bakeng sa ho tiisa le ho e kenya ka 36°C bakeng sa 24h.
Sehlahisoa se thibelang mocheso oa coliform/fecal coliform ho 44.5°C bakeng sa 24h e mosehla ebile e ntle.
4. Tlhaloso ea liphetho:
colorless = mpe
Yellow checkered = positive total coliforms
Yellow + fluorescent grid = Escherichia coli positive reference MPN table count